Cuihong Wan Ph.D.


College of  life sciences, Central China Normal University

E-mail: wan.cuihong@foxmail.com

Work Experience:

2015.10 每 present     Central China Normal University, Professor

2014.10 每 2015.6     University of Texas at Austin, Research Fellow
   Collaborator supervisor: Prof. Edward Marcotte

2009.10 每 2014.7     University of Toronto, Postdoctoral Fellow
    Collaborator supervisor: Prof. Andrew Emili

Educational Background:

Doctor of Natural Science    Changchun Institute of Applied Chemistry Chinese Academy of Sciences
(2004.9 每 2009.7)   (Supervisor: Prof. Shuying Liu)

Bachelor of Engineering     Huazhong University of Science and Technology
(2000.9 每 2004.6)   

Research Projects:

  1. Plant soluble protein complexes (2014.10 - now)
  2. Metabolism compare between human and yeast (2015-now)
  3. Quantitative proteomic assessment of protein complexes across metazoan (2009 - 2014)
  4. Functional profiling of mutant and null TP53 cells (2012-2014)
  5. Identify phosphorylation dynamics during sea urchin egg activation (2011-2013)
  6. Protein label free quantification (2010-2012)
  7. Non-covalent Interactions of Small Molecules and Triplex DNAs (2006 每 2009, PhD Thesis)
  8. Epitope Mapping Using Immunoaffinity Mass Spectrometry (2004 - 2005)

Publications:

Full papers:

  1. Wan C#, Borgeson B#, Phanse S, Tu F, Drew K, Clark G, Xiong X, Kagan O, Kwan J, Berzginov A, Chessman K, Pal S, Cromar G, Papoulas O, Ni Z, Boutz DR, Guo X, Malty RH, Sarov M, Greenblatt J, Babu M, Derry B, Tillier E, Wallingford JB, Parkinson J, Marcotte EM, Emili A. Panorama of ancient metazoan macromolecular complexes. Nature, 2015, 525(7569):339-44 (# Co-first Author)
  2. Guo H, Garcia-Vedrenne AE, Isserlin R, Lugowski A, Morada A, Sun A, Miao Y, Kuzmanov U, Wan C, Ma H, Foltz K, Emili A. Phosphoproteomic network analysis in the sea urchin Strongylocentrotus purpuratus reveals new candidates in egg activation. Proteomics. 2015, doi: 10.1002/pmic
  3. Ni Z, Xu C, Guo X, Hunter GO, Kuznetsova OV, Tempel W, Marcon E, Zhong G, Guo H, Kuo WH, Li J, Young P, Olsen JB, Wan C, Loppnau P, El Bakkouri M, Senisterra GA, He H, Huang H, Sidhu SS, Emili A, Murphy S, Mosley AL, Arrowsmith CH, Min J, Greenblatt JF. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nat Struct Mol Biol. 2014, 21(8): 686-95.
  4. Wan C, Liu J, Fong V, Lugowski A, Stoilova S, Bethune-Waddell D, Borgeson B, Havugimana PC, Marcotte EM, Emili A. ComplexQuant: High-throughput computational pipeline for the global quantitative analysis of endogenous soluble protein complexes using high resolution protein HPLC and precision label-free LC/MS/MS. J Proteomics. 2013, 81:102-111.
  5. Havugimana PC, Hart GT, Nepusz T, Yang H, Turinsky AL, Li Z, Wang PI, Boutz DR, Fong V, Phanse S, Babu M, Craig SA, Hu P, Wan C, Vlasblom J, Dar VU, Bezginov A, Clark GW, Wu GC, Wodak SJ, Tillier ER, Paccanaro A, Marcotte EM, Emili A. A census of human soluble protein complexes. Cell. 2012,150(5):1068-1081.
  6. Xu N, Yang H, Cui M, Wan C, Liu S. High-performance liquid chromatography-electrospray ionization-mass spectrometry ligand fishing assay: a method for screening triplex DNA binders from natural plant extracts. Anal. Chem. 2012, 84(5):2562-2568.
  7. Mak AB, Ni Z, Hewel JA, Chen GI, Zhong G, Karamboulas K, Blakely K, Smiley S, Marcon E, Roudeva D, Li J, Olsen JB, Wan C, Punna T, Isserlin R, Chetyrkin S, Gingras AC, Emili A, Greenblatt J, Moffat J. A lentiviral functional proteomics approach identifies chromatin remodeling complexes important for the induction of pluripotency. Mol Cell Proteomics. 2010, 9(5):811-23.
  8. Wan C, Cui M, Song F, Liu Z, Liu S. A Study of the Non-Covalent Interaction between Flavonoids and DNA Triplexes by Electrospray Ionization Mass Spectrometry. Int. J Mass Spectrom 2009, 283:48-55.
  9. Wan C, Cui M, Song F, Liu Z, Liu S. Evaluate Effects of Bivalent Cations on the Formation of Purine-rich Triple Helix DNA by ESI-FT-MS. J Am Soc Mass Spectrom. 2009, 20(7):1281-1286
  10. Wan C, Guo X, Song F, Liu Z, Liu S. Interactions of Mitoxantrone with Duplex and Triplex DNA Studied by Electrospray Ionization Mass Spectrometry. Rapid Commun Mass Spectrom. 2008, 22: 4043?4048.
  11. Wan C, Guo X, Liu Z, Liu S. Studies of the Intermolecular DNA Triplexes of C+?GC and T?AT Triplets by Electrospray Ionization Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry. J Mass Spectrom. 2008, 43(2):164-172.
  12. Wan C, Li G, Liu Z, Liu S. Characterization Epitope of the Beta(2)-microglobulin with Immunoaffinity Mass Spectrometry. Acta Chimica Sinica. 2008, 66(13):1572-1576

Presentations:

  1. Wan C, Borgeson B, Phanse S, Tu F, Drew K, Clark G, Xiong X, Kagan O, Kwan J, Berzginov B, Chessman K, Papoulas O, Boutz DR, Tillier E, Wallingford JB, Parkinson J, Emili A, Marcotte EM. Protein 2. complexes conserved across metazoan. Systems Biology: Networks, New York, USA, March17-21, 2015, Poster
  2. Wan C, Havugimana PC, Lugowski A, Foltz K, Emili A. Large-scale LC-MS-based profiling of soluble protein complexes. 19th international Mass Spectrometry Conference. Kyoto, Japan, Sep.15-21, 2012, Poster.
  3. Wan C, Liu J, Emili A. Label-free quantification base on precursor peak isotope pattern mapping for evaluate dynamic of protein complexes. 59th ASMS Conference on Mass Spectrometry and Allied Topics. Denver, USA, June 5-9, 2011. Poster.
  4. Wan C, Song F, Cui M, Liu Z, Liu S. Studies on the Interaction of Mitoxantrone with Duplex and Triplex DNA by ESI-FTICR-MS. Journal of Chinese Mass Spectrometry Society. 2008, 29 (supplement): 204-205. 8th China Mass Spectrometry Society Congress, Hailan, China. Poster.
  5. Wan C, Guo X, Liu Z, Liu S. Study of the Intermolecular DNA Triplexes by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. The 12th International Beijing Conference and Exhibition on Instrumental Analysis (12th BCEIA). Oct. 18-21, 2007. Oral presentation.
  6. Wan C, Liu Z, Liu S. Studies on the Interaction of Netropsin with Duplex and Triplex DNA by ESI-MS. Journal of Chinese Mass Spectrometry Society. 2007, 28 (supplement (organic)): 48-49. China Organic Mass Spectrometry 14th National Congress, shanghai, China. Poster.